ATP2A2 抗体 (AA 498-510)
Quick Overview for ATP2A2 抗体 (AA 498-510) (ABIN7042954)
抗原
See all ATP2A2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 498-510
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原理
- A Rabbit Polyclonal Antibody to SERCA2 Pump
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特异性
- 2nd cytoplasmic loop
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Mouse,human - identical
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产品特性
- Anti-SERCA2 Antibody is directed against an epitope of rat SERCA2. Anti-SERCA2 Antibody (ABIN7042954, ABIN7044151 and ABIN7044152) can be used in western blot and immunocytochemistry applications and was designed to recognize SERCA2 from rat, mouse and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: CTPNKPSRTSMSK, corresponding to amino acid residues 498-510 of rat SERCA2
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亚型
- IgG
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anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) (AA 111-253) antibody
ATP2A2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) (AA 315-756) antibodyATP2A2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) (N-Term) antibodyATP2A2 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) (AA 314-756) antibodyATP2A2 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) (Internal Region) antibodyVerified ATP2A2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) (C-Term) antibodyATP2A2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) (Internal Region) antibodyATP2A2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) antibodyATP2A2 适用: 人 WB, IP 宿主: 兔 Monoclonal unconjugated
anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) (C-Term) antibodyATP2A2 适用: 人, 小鼠, 大鼠, 兔, Cow, 犬, 豚鼠, 马, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) (C-Term) antibodyATP2A2 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7234817)
Blocking Peptide: (ABIN7234817)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- ATP2A2 (ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2))
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别名
- ATP2A2
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背景
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Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, Calcium pump 2, ATP2A2,Three Ca2+ ATPases have been described in mammalian cells. They are located in the plasma membrane, endoplasmic reticulum or the Golgi apparatus. SERCA pumps are located in both the endoplasmic reticulum and in the Golgi membranes. They are known to transport two Ca2+ molecules per hydrolysis of one ATP1. Their structure includes ten transmembrane domains and their main role is to remove cytoplasmic Ca2+ ions in order to promote muscle relaxation1.In mammals three genes encode three SERCA pumps. Each transcript undergoes tissue-dependent alternative splicing. SERCA1a and 1b are expressed in adult and neonatal skeletal muscle respectively. SERCA2a is also expressed in skeletal muscle, while SERCA2b is ubiquitously expressed. SERCA3 is expressed in a limited number of non-muscle cells2. Although all SERCAs are regulated, SERCA2b undergoes extensive regulation at the protein level, such as protein-protein interaction, phosphorylation and glycosylation1.SERCA2 catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization3,4.
Alternative names: SERCA2, Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, Calcium pump 2, ATP2A2 -
基因ID
- 29693
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NCBI登录号
- NM_001681
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UniProt
- P11507
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途径
- Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Ribonucleoside Biosynthetic Process
抗原
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