Aquaporin 4 抗体 (Intracellular) (Atto 594)
Quick Overview for Aquaporin 4 抗体 (Intracellular) (Atto 594) (ABIN7042940)
抗原
See all Aquaporin 4 (AQP4) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 300-314, Intracellular
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原理
- A Rabbit Polyclonal Antibody to Aquaporin 4 Conjugated to the Fluorescent Dye ATTO-594
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特异性
- Intracellular, C-terminus
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交叉反应
- 小鼠, 大鼠
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预测反应
- human - 13,15 amino acid residues identical,Mouse - 14
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产品特性
- Anti-Aquaporin 4 (AQP4) (300-314) Antibody (ABIN7042939, ABIN7045209 and ABIN7045210) is a highly specific antibody directed against an epitope of rat AQP4. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize the Aquaporin 4 channel from rat, mouse, and human samples. \nAnti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody (ABIN7042940) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)HVIDIDRGDEKKGKD, corresponding to amino acid residues 300-314 of rat AQP4
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亚型
- IgG
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anti-Aquaporin 4 (AQP4) (AA 271-323) antibody
AQP4 适用: 人, 小鼠, 大鼠 WB, ICC, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 4 (AQP4) (AA 178-317) antibodyAQP4 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C9 unconjugated
anti-Aquaporin 4 (AQP4) (AA 244-323) antibodyAQP4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 4 (AQP4) (AA 295-323), (C-Term) antibodyAQP4 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 4 (AQP4) (AA 253-323) antibodyAQP4 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 4 (AQP4) antibodyMicroarray verified AQP4 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB430199 unconjugated
anti-Aquaporin 4 (AQP4) antibodyMicroarray verified AQP4 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB287702 unconjugated
anti-Aquaporin 4 (AQP4) antibodyAQP4 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Aquaporin 4 (AQP4) (AA 258-274) antibodyAQP4 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 4 (AQP4) (AA 178-317) antibodyAQP4 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:80-1:100
Application Dilutions Western blot wb: N/A
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 50 μL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Aquaporin 4 (AQP4)
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别名
- AQP4
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背景
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WCH4, Mercurial-insensitive water channel,Aquaporin 4 (AQP-4) belongs to a family of membrane proteins that allow passage of water and certain solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).The aquaporins can be divided into two functional groups based on their permeability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-4 together with AQP-1, AQP-2 and AQP-5 belong to the first group1. Little is known about the function of the two newest members, AQP-11 and AQP-12.The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores1.AQP-4 is the major membrane water channel in the central nervous system. The channel is expressed in astrocyte foot processes in direct contact with capillary vessels in the brain suggesting a role in water transport under normal and pathological conditions. Indeed, transgenic mice lacking AQP-4 have reduced brain swelling and improved neurological outcome following water intoxication and focal cerebral ischemia. In contrast, brain swelling and clinical outcome are worse in AQP-4-null mice in models of vasogenic (fluid leak) edema caused by freeze-injury and brain tumor, probably due to impaired AQP-4-dependent brain water clearance2.In addition, it has been recently shown that neuromyelitis optica (NMO), an inflammatory demyelinating disease that selectively affects optic nerves and spinal cord, is caused by the development of an autoantibody directed against AQP-43.
Alternative names: Aquaporin 4 (AQP4), WCH4, Mercurial-insensitive water channel -
基因ID
- 25293
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UniProt
- P47863
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途径
- Sensory Perception of Sound
抗原
-
