ADAM22 抗体 (Extracellular)
Quick Overview for ADAM22 抗体 (Extracellular) (ABIN7042881)
抗原
See all ADAM22 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 715-729, Extracellular
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原理
- A Rabbit Polyclonal Antibody to ADAM22
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特异性
- Extracellular, N-terminus
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Rat,human - identical
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产品特性
- Anti-ADAM22 (extracellular) Antibody (ABIN7042881, ABIN7044724 and ABIN7044725) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize ADAM22 from rat, mouse, and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)HNDDAKTGITLSGNG, corresponding to amino acid residues 715-729 of mouse ADAM22
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亚型
- IgG
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anti-ADAM Metallopeptidase Domain 22 (ADAM22) (AA 370-582) antibody
ADAM22 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 22 (ADAM22) (AA 1-342) antibodyADAM22 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 22 (ADAM22) (AA 298-347) antibodyADAM22 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, Bat, 猴, Pig, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 22 (ADAM22) (AA 223-400) antibodyADAM22 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 22 (ADAM22) (N-Term) antibodyADAM22 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 22 (ADAM22) antibodyADAM22 适用: 小鼠, 大鼠 WB 宿主: 豚鼠 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7234619)
Blocking Peptide: (ABIN7234619)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- ADAM22 (ADAM Metallopeptidase Domain 22 (ADAM22))
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别名
- ADAM22
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背景
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Disintegrin and metalloproteinase domain-containing protein 22, ADAM metallopeptidase domain 22, MDC2,ADAMs (a disintegrin and metalloproteinases) are multi-domain transmembrane glycoproteins with diverse roles in physiology and disease. Notably, 8 of 21 ADAMs lack functional metalloproteinase domains and are implicated in protein-protein interactions instead of membrane protein ectodomain shedding.ADAM22 is a non-proteinase which acts as a post synaptic receptor for the secreted neurotransmission modulator LGI-1 at neural synapses. ADAM22 is a compact four-leaf clover with the metalloproteinase-like domain (Domain M) held in the concave face of a rigid module formed by the disintegrin (Domain D), cysteine-rich (Domain C), and epidermal growth factor-like domains (Domain E). The largest domain in the four-leaf clover, domain M, is distal to the cell membrane. Following domain M, domain D and domain C zigzag to domain E in a compact, but not extended fashion. A 15-amino acid linker, leads the C terminus of domain E to the membrane. The loss of metalloproteinase activity is ensured by the absence of critical catalytic residues, the filling of the substrate groove, and the steric hindrance by the cysteine-rich domain. The extracellular domain of ADAM22 interacts with LGI-1, whereas its cytoplasmic PDZ-binding motif recruits PSD-95. The link of ADAM22 and LGI-1 to AMPA receptors establishes their roles in glutamate neurotransmission1.Mutations that impair LGI-1 binding to ADAM22 are implicated in the pathogenesis of Autosomal dominant lateral temporal epilepsy (ADTLE), a focal epilepsy syndrome characterized by focal seizures with prominent auditory or aphasic symptoms, normal magnetic resonance imaging, and usually benign evolution2.
Alternative names: ADAM22, Disintegrin and metalloproteinase domain-containing protein 22, ADAM metallopeptidase domain 22, MDC2 -
基因ID
- 11496
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NCBI登录号
- NM_021723
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UniProt
- Q9R1V6
抗原
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