GATA1 抗体 (C-Term)
Quick Overview for GATA1 抗体 (C-Term) (ABIN7013869)
抗原
See all GATA1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- GATA1
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序列
- PTTSTTVVAP LSS
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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应用备注
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Western Blot: Approx 50 kDa band observed in nuclear lysates of cell line K562 (calculated MW of 42.8 kDa according to NP_002040.1). The observed molecular weight corresponds to different antibodies from other sources and was successfully blocked by incuba
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.
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限制
- 仅限研究用
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))
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别名
- GATA1
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背景
- GATA1, GATA binding protein 1 (globin transcription factor 1), GF1, NFE1, ERYF1
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基因ID
- 2623
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途径
- Cellular Response to Molecule of Bacterial Origin
抗原
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