EDF1 抗体 (N-Term)
Quick Overview for EDF1 抗体 (N-Term) (ABIN7013866)
抗原
See all EDF1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- N-Term
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原理
- EDF1 / MBF1
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序列
- AESDWDTVTV LRK
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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应用备注
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Immunohistochemistry: Paraffin embedded Human Pancreas and Brain (Cortex). Recommended concentration: 3.75 μg/mL.
Western Blot: Approx 19 kDa band observed in nuckear lysates of cell line HEK293, approx. 18 kDa in nuckear lysates of cell line HeLa, and approx 17 kDa in nuckear lysates of cell line NIH3T3 (calculated MW of 16.4 kDa according to Human NP_003783.1 and Mous
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10u
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限制
- 仅限研究用
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))
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别名
- EDF1
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背景
- EDF1, MBF1, EDF-1, MGC9058, endothelial differentiation-related factor 1, multiprotein bridging factor 1
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基因ID
- 8721, 59022
抗原
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