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EDF1 抗体 (N-Term)

This anti-EDF1 antibody is a 山羊 多克隆 antibody detecting EDF1 in WB, IHC, ELISA, IF 和 FACS. Suitable for 人 和 小鼠.
产品编号 ABIN7013866
发货至: 中国

Quick Overview for EDF1 抗体 (N-Term) (ABIN7013866)

抗原

See all EDF1 抗体
EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

适用

  • 18
  • 7
  • 7
  • 7
  • 6
  • 5
  • 4
  • 3
  • 3
  • 3
  • 1
人, 小鼠

宿主

  • 17
  • 2
  • 1
山羊

克隆类型

  • 19
  • 1
多克隆

标记

  • 20
This EDF1 antibody is un-conjugated

应用范围

  • 20
  • 10
  • 5
  • 5
  • 5
  • 3
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    原理

    EDF1 / MBF1

    序列

    AESDWDTVTV LRK

    交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Pancreas and Brain (Cortex). Recommended concentration: 3.75 μg/mL.

    Western Blot: Approx 19 kDa band observed in nuckear lysates of cell line HEK293, approx. 18 kDa in nuckear lysates of cell line HeLa, and approx 17 kDa in nuckear lysates of cell line NIH3T3 (calculated MW of 16.4 kDa according to Human NP_003783.1 and Mous

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10u

    限制

    仅限研究用
  • 缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

    别名

    EDF1

    背景

    EDF1, MBF1, EDF-1, MGC9058, endothelial differentiation-related factor 1, multiprotein bridging factor 1

    基因ID

    8721, 59022
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