SCRIB 抗体 (Internal Region)
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Quick Overview for SCRIB 抗体 (Internal Region) (ABIN7013862)
抗原
See all SCRIB 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- SCRIB / Scribble
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序列
- PEGPGKEKEL PGQ
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特异性
- This antibody is expected to recognize both reported isoforms (NP_874365.3, NP_056171.3).
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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anti-Scribbled Homolog (SCRIB) antibody
SCRIB 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Scribbled Homolog (SCRIB) (AA 382-410), (N-Term) antibodySCRIB 适用: 人 WB 宿主: 兔 Polyclonal RB39490 unconjugated
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应用备注
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Western Blot: Approx 200-250 kDa band observed in lysates of cell lines HeLa, HepG2 and U2OS (calculated MW of 178 kDa according to NP_874365.3). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3 μg/mL. Primary inc
Peptide ELISA: antibody detection limit dilution 1:1000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cell junctions of MCF7 cells. Recommended concentration: 10μg/ml.
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限制
- 仅限研究用
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- SCRIB (Scribbled Homolog (SCRIB))
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别名
- SCRIB
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背景
- SCRIB, scribbled homolog (Drosophila), CRIB1, SCRB1, SCRIB1, Vartul, scribble
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基因ID
- 23513
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途径
- Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Tube Formation, Synaptic Vesicle Exocytosis, Asymmetric Protein Localization
抗原
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