SSTR3 抗体
(1 validation)Quick Overview for SSTR3 抗体 (ABIN7242716)
抗原
See all SSTR3 抗体适用
宿主
克隆类型
标记
应用范围
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产品特性
- Polyclonal Antibody
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纯化方法
- Affinity purification
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免疫原
- Synthetic peptide of human SSTR3
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亚型
- IgG
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anti-Somatostatin Receptor 3 (SSTR3) (AA 1-43) antibody
SSTR3 适用: 人, 大鼠 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 7H8E5 unconjugated
anti-Somatostatin Receptor 3 (SSTR3) (AA 1-43) antibodySSTR3 适用: 人, 大鼠 宿主: 小鼠 Monoclonal unconjugated
anti-Somatostatin Receptor 3 (SSTR3) antibodySSTR3 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Somatostatin Receptor 3 (SSTR3) (C-Term) antibodySSTR3 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Somatostatin Receptor 3 (SSTR3) (AA 250-350) antibodySSTR3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- IHC 1:50-1:200
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限制
- 仅限研究用
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- by
- Idevall Lab, Department of Medical Cell Biology, Uppsala University
- No.
- #103937
- 日期
- 2019.06.18
- 抗原
- SSTR3
- Lot Number
- DK1445
- Method validated
- Immunofluorescence
- Positive Control
Pancreatic beta cell line MIN6, stained for acetylated tubulin
- Negative Control
- Notes
ABIN7242714 specifically labels the targeted antigen in mouse insulinoma cells in IF. No signal was detected in sample negative control tissue and the secondary antibody only control.
- Primary Antibody
- ABIN7242714
- Secondary Antibody
- Full Protocol
- Grow MIN6 cells (gift from Prof. Yamamura) in DMEM (ThermoFisher, 41965039, lot 2071491) supplemented with FBS (ThermoFisher, 10500064, lot 08Q6291K) and Penicilin-Streptomycin (ThermoFisher, 15140122, lot 2051356), at 37 °C and 5% CO2 in 2 mL on glass coverslips.
- Starve cells for 48 h in serum free medium to promote ciliogenesis.
- Wash cells with 37 °C DPBS (ThermoFisher, 14190094, lot 2062255).
- Fix cells on coverslips in 4% PFA for 2 min at RT.
- Wash cells 3x for 2 min with PBS.
- Permeabilize cells in PBS containing 0.1% Triton-X for 2 min at RT.
- Block non-specific binding with PBS containing 1% serum for 1 h at RT.
- Incubate cells with primary
- rabbit anti-SSTR3 antibody (antibodies-online, ABIN7242714, lot DK1445) and
- mouse anti-actetylated tubulin (Sigma-Aldrich, T7451, lot 078M4842V) diluted 1:100 in 1% BSA PBS for 2 h at RT.
- Wash cells 3x for 2 min with PBS.
- Incubate cells with secondary
- goat anti-rabbit AF488-conjugated antibody (ThermoFisher, A11034, lot 2069632) and
- goat anti-mouse AF568-conjugated antibody (ThermoFisher, A11004, lot 927620) diluted 1:200 in 1% BSA PBS for 1 h at RT.
- Wash cells 2x for 2 min with PBS.
- Mount coverslips on glass slides in ProLong Gold antifade reagent with DAPI (ThermoFisher, P36935, lot 2086914).
- Image acquisition with Zeiss LSM780, 60x magnification.
- Experimental Notes
Our results are consistent with previous studies that have revealed the exclusive expression of SSTR3 in the cilia of mouse pancreatic beta-cells (Iwanaga et al. (2011)).
生效 #103937 (Immunofluorescence)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Staining of MIN6 cells with and antibody directed against acetylated tubulin (red, a bona fide marker of the cilium), ABIN7242714 (green) and DAPI (blue). Composite image shows the overlap of red and green staining in the cilium.]()
Staining of MIN6 cells with and antibody directed against acetylated tubulin (red, a bona fide marker of the cilium), ABIN7242714 (green) and DAPI (blue). Composite image shows the overlap of red and green staining in the cilium.
![Fluorescence profiles measured along the cilium of the channels corresponding to acetylated tubulin (red) and SSTR3 (green) confirming enrichment of the receptor in the medial portion of the cilium.]()
Fluorescence profiles measured along the cilium of the channels corresponding to acetylated tubulin (red) and SSTR3 (green) confirming enrichment of the receptor in the medial portion of the cilium.
Full Methods -
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状态
- Liquid
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浓度
- 0.4 mg/mL
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缓冲液
- PBS with 0.05 % sodium azide and 50 % glycerol, PH7.4
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C. Avoid freeze / thaw cycles.
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- SSTR3 (Somatostatin Receptor 3 (SSTR3))
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别名
- SSTR3
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背景
- Somatostatin acts at many sites to inhibit the release of many hormones and other secretory proteins. The biological effects of somatostatin are probably mediated by a family of G protein-coupled receptors that are expressed in a tissue-specific manner. SSTR3 is a member of the superfamily of receptors having seven transmembrane segments and is expressed in highest levels in brain and pancreatic islets. SSTR3 is functionally coupled to adenylyl cyclase.
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NCBI登录号
- NP_001042
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UniProt
- P32745
抗原
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