Aconitase 1 抗体 (AA 251-443)
Quick Overview for Aconitase 1 抗体 (AA 251-443) (ABIN7438541)
抗原
See all Aconitase 1 (ACO1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 251-443
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原理
- Polyclonal Antibody to Aconitase 1 (ACO1)
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特异性
- The antibody is a rabbit polyclonal antibody raised against ACO1. It has been selected for its ability to recognize ACO1 in immunohistochemical staining and western blotting.
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交叉反应
- 小鼠, Pig, 大鼠
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纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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免疫原
- Recombinant Aconitase 1 (ACO1) corresdonding to Pro251~Pro443 (Accession # P21399)
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亚型
- IgG
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应用备注
- Western blotting: 1-5 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Flow cytometry:20 μg/mL,Optimal working dilutions must be determined by end user.,
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说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 500 μg/mL
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缓冲液
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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有效期
- 24 months
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- Aconitase 1 (ACO1)
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别名
- ACO1
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背景
- IREB1, IRP1, IREBP, Iron regulatory protein 1, Iron-responsive element-binding protein 1, Cytoplasmic aconitate hydratase, Citrate hydro-lyase, Ferritin repressor protein
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UniProt
- P21399
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途径
- Transition Metal Ion Homeostasis
抗原
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