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- 抗原
- VEGF 165
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抗原表位
- AA 28-191
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
- 原理
- Polyclonal Antibody to Vascular Endothelial Growth Factor 165 (VEGF165)
- 特异性
- The antibody is a rabbit polyclonal antibody raised against VEGF165. It has been selected for its ability to recognize VEGF165 in immunohistochemical staining and western blotting.
- 纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- 免疫原
- Recombinant Vascular Endothelial Growth Factor 165 (VEGF165) corresdonding to Pro28~Arg191 with N-terminal His Tag
- 亚型
- IgG
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anti-VEGF 165 (AA 28-191) antibody
适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal A2 unconjugated
anti-VEGF 165 antibody适用: 人 WB, IHC, ELISA, Neut, RIA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western blotting: 0.5-2 μg/mL
1:500-2000 Immunohistochemistry: 5-20 μg/mL
1:50-200 Immunocytochemistry: 5-20 μg/mL
1:50-200 Optimal working dilutions must be determined by end user.
- 说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- 有效期
- 24 months
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- 抗原
- VEGF 165
- 别名
- Vascular Endothelial Growth Factor 165
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