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Histone H4 抗体 (AA 2-103)

Name: Histone H4 抗体 (AA 2-103)
Brand: antibodies-online
SKU: ABIN7440314
Availability: OutOfStock
Rating: 5 (1 reviews)

This anti-Histone H4 antibody is a 兔多克隆 antibody detecting Histone H4 in WB, IHC, ICC 和 IP. Suitable for 人, 小鼠, 大鼠, Pig, Cow, 小鸡, Cat, 犬, 豚鼠, 马和猕猴. Independently validated for use in Immunohistochemistry.

Immunohistochemistry validation image for anti-Histone H4 (AA 2-103) antibody (ABIN7440314) (Histone H4 抗体 (AA 2-103))

IHC

Detection of Recombinant H4, Human using Polyclonal Antibody to Histone H4 (H4) (Histone H4 抗体 (AA 2-103))

2 images

(1 validation)

产品编号 ABIN7440314

发货至: 中国

Quick Overview for Histone H4 抗体 (AA 2-103) (ABIN7440314)

抗原

See all Histone H4 抗体

Histone H4

适用

人, 小鼠, 大鼠, Pig, Cow, 小鸡, Cat, 犬, 豚鼠, 马, 猕猴

宿主

兔

克隆类型

多克隆

标记

This Histone H4 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Discover our top product Histone H4 Primary Antibody

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AA 2-103

原理

Polyclonal Antibody to Histone H4 (H4)

特异性

The antibody is a rabbit polyclonal antibody raised against H4. It has been selected for its ability to recognize H4 in immunohistochemical staining and western blotting.

交叉反应

Cat, 小鸡, Cow, 犬, 豚鼠, 马, 人, 小鼠, Pig, 兔, 大鼠, 猕猴

纯化方法

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

免疫原

Recombinant Histone H4 (H4) corresdonding to Ser2~Gly103 with N-terminal His and GST Tag

亚型

IgG
anti-Histone H4 (acLys12) antibody
适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated

anti-Histone H4 (3meLys20) antibody
适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Histone H4 (acLys5) antibody
适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated

anti-Histone H4 (2meLys20) antibody
适用: 人 WB, IHC, IF, IP, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H4 (3meLys20) antibody
适用: 人 WB, IHC, IF, DB 宿主: 兔 Polyclonal unconjugated

anti-Histone H4 (acLys8) antibody
适用: 人 WB, IHC, IF, IP, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H4 (acLys12) antibody
适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Histone H4 (acLys8) antibody
适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Histone H4 (acLys16) antibody
适用: 人, Saccharomyces cerevisiae, Drosophila melanogaster WB, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H4 (meArg3) antibody
适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

应用备注

Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

说明

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

限制

仅限研究用
生效 #104631 (Immunohistochemistry)

by

Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin

No.

#104631

日期

2025.08.04

抗原

Histone H4

Lot Number

A20250716116

Method validated

Immunohistochemistry

Positive Control

Negative Control

Control slices were processed for each experimental procedure, omitting the primary antibody. In this case, overnight incubation was only performed using a diluent solution.

Notes

Validation Images

Histone H4 immunoreactive cells in the cerebral cortex and hippocampus of an adult CD1 mouse. Antibody dilution: 1:25. Magnification: 63x.
Full Methods
Primary Antibody

ABIN7440314

Secondary Antibody

anti-rabbit Alexa Fluor 488 (Invitrogen A11008, lot # 3112790)

Full Protocol

Sample analyzed: adult (9 months old) CD1 mouse brain.
Paraffin-embedded slices (7µm thick) were deparaffinized and rehydrated through a graded series of alcohols and distilled water (dH2O).
Sections were blocked in ovalbumin 1% 1 hour at room temperature.
Antigen retrieval was performed in all the sections by microwave treatment (1 minute at 750W + 1 minute at 250W in Citrate buffer pH 6).
3x5 minutes washing in 0.01M PBS.
Sections were incubated with the primary antibody at the following dilutions of 1:25/1:50/1:100/1:200, overnight at room temperature (primary antibody was diluted in PLL/BSA/PBS diluent).
3x5 minutes washing in 0.01M PBS.
Incubation with the anti-rabbit secondary antibody 1:400 in PLL/BSA/PBS diluent, 1 hour at room temperature.
3x5 minutes washing in 0.01M PBS.
Nuclear counterstaining was performed with 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) 1µg/mL, 2 minutes.
2x5 minutes washing in 0.01M PBS.
Specimens were then mounted in Fluoroshield mounting medium (Sigma).
Images were acquired with a Leica DM 6000B fluorescence microscope with a digital camera at 63x magnification.

Experimental Notes

Passed. The antibody works in IHC-P at concentrations from 1:25-1:50.
状态

Liquid

浓度

0.33 mg/mL

缓冲液

PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

有效期

24 months
抗原

Histone H4