IL-4 抗体 (AA 26-147) (APC)
Quick Overview for IL-4 抗体 (AA 26-147) (APC) (ABIN7436810)
抗原
See all IL-4 (IL4) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 26-147
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原理
- Polyclonal Antibody to Interleukin 4 (IL4)
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特异性
- The antibody is a cavia polyclonal antibody raised against IL4. It has been selected for its ability to recognize IL4 in immunohistochemical staining and western blotting.
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纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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免疫原
- Recombinant Interleukin 4 (IL4) corresdonding to Arg26~Ser147 (Accession # Q9MZR8)
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亚型
- IgG
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应用备注
- Western blotting: 0.5-2 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.
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说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 500 μg/mL
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缓冲液
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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有效期
- 24 months
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- IL-4 (IL4) (Interleukin 4 (IL4))
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别名
- Interleukin 4
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背景
- BSF1, BCGF1 ,B Cell Stimulatory Factor 1, Lymphocyte stimulatory factor 1, B Cell Growth Factor, Binetrakin, Pitrakinra
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UniProt
- Q9MZR8
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
抗原
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