Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
特异性
AJAP1 antibody is human, mouse and rat reactive.
纯化方法
AJAP1 antibody is affinity chromatography purified via peptide column.
免疫原
AJAP1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human AJAP1. The immunogen is located within amino acids 290 - 340 of AJAP1.
AJAP1 antibody can be used for detection of AJAP1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ,g/mL.
Antibody validated: Western Blot in rat samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
AJAP1 antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
AJAP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
抗原
AJAP1
(Adherens Junctions Associated Protein 1 (AJAP1))
The Adherens Junction Associated Protein 1 (AJAP1) plays a key role in cell adhesion and cell migration (1). It is a transmembrane protein found on the basolateral surface of epithelium and expressed by multiple types of epithelium (1,2). AJAP1 interacts with E-Cadherin and appears to promote the internalization of E-Cadherin upon growth factor stimulation (3). This facilitates the dissolution of adherens junctions with an increase in cell motility (3,4).