WWC1 antibody is human, mouse and rat reactive. At least three isoforms of WWC1 are known to exist, this antibody will detect all three isoforms.
纯化方法
WWC1 antibody is affinity chromatography purified via peptide column.
免疫原
WWC1 antibody was raised against an 18 amino acid peptide near the amino terminus of human WWC1. The immunogen is located within amino acids 60 - 110 of WWC1.
WWC1
适用: 人, 小鼠, 大鼠
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
Alexa Fluor 647
应用备注
WWC1 antibody can be used for detection of WWC1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
WWC1 antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
WWC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
The WW and C2 domain containing 1 (WWC1) protein, also known as KIBRA, possesses two WW domains and an internal C2-like domain (1). WWC1 was originally identified as a memory performance-associated protein in humans (2) and has recently been shown to be a novel regulator of the Hippo pathway (3). WWC1 is phosphorylated by the mitotic kinases Aurora-A and -B (4), and in turn activates the Aurora kinases and is required for precise chromosome alignment during mitosis (5).