E2F3 抗体 (AA 150-200)
Quick Overview for E2F3 抗体 (AA 150-200) (ABIN6992046)
抗原
See all E2F3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 150-200
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特异性
- E2F3 antibody is human and mouse reactive. At least three isoforms of E2F3 are known to exist, this antibody will detect only the largest isoform. This antibody is predicted to not cross-react with other members of the E2F transcription factor family.
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纯化方法
- E2F3 antibody is affinity chromatography purified via peptide column.
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免疫原
- E2F3 antibody was raised against a 15 amino acid peptide near the center of human E2F3. The immunogen is located within amino acids 150 - 200 of E2F3.
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亚型
- IgG
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anti-E2F3 (AA 336-425) antibody
适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 5F7 unconjugated
anti-E2F3 (C-Term) antibody适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-E2F3 (AA 381-465) antibody适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-E2F3 (C-Term) antibody适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, Bat, Pig, Hamster WB, IHC (p), IHC 宿主: 兔 Polyclonal unconjugated
anti-E2F3 (acLys168) antibody适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-E2F3 (AA 200-300) antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-E2F3 (AA 1-108) antibody适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-E2F3 (AA 1-133) antibody适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-E2F3 (C-Term) antibodyVerified 适用: 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-E2F3 (AA 1-133) antibody适用: 人 ELISA 宿主: 小鼠 Monoclonal 3C11 unconjugated
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应用备注
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E2F3 antibody can be used for detection of E2F3 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- E2F3 antibody is supplied in PBS containing 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,4 °C
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储存方法
- E2F3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
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- E2F3
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背景
- The E2F transcription factor 3 (E2F3) is a member of a small family of transcription factors that function through binding of DP interaction partner proteins. E2F3 recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle (1). Like the related E2F1 and E2F2, E2F3 is essential for cellular proliferation and progression through the cell cycle (2). Altered copy number and activity of this gene have been observed in a number of human cancers (3).
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分子量
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Predicted: 51 kDa
Observed: 56 kDa -
基因ID
- 1871
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NCBI登录号
- NP_001940
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UniProt
- O00716
抗原
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