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USP25 抗体 (AA 470-520)

This anti-USP25 antibody is a 兔 多克隆 antibody detecting USP25 in WB, ELISA, IHC (p) 和 IF. Suitable for 人 和 小鼠.
产品编号 ABIN6991995
发货至: 中国

Quick Overview for USP25 抗体 (AA 470-520) (ABIN6991995)

抗原

See all USP25 抗体
USP25 (Ubiquitin Specific Peptidase 25 (USP25))

适用

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人, 小鼠

宿主

  • 29
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克隆类型

  • 29
  • 1
多克隆

标记

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This USP25 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
  • 抗原表位

    • 8
    • 8
    • 7
    • 5
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    • 1
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    • 1
    • 1
    AA 470-520

    特异性

    USP25 antibody is human and mouse reactive. Multiple isoforms of USP25 are known to exist.

    纯化方法

    USP25 antibody is affinity chromatography purified via peptide column.

    免疫原

    USP25 antibody was raised against a 17 amino acid peptide near the center of human USP25. The immunogen is located within amino acids 470 - 520 of USP25.

    亚型

    IgG
  • 应用备注

    USP25 antibody can be used for detection of USP25 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.

    Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    USP25 Antibody is supplied in PBS containing 0.02 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C,4 °C

    储存方法

    USP25 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
  • 抗原

    USP25 (Ubiquitin Specific Peptidase 25 (USP25))

    别名

    USP25

    背景

    USP25 (ubiquitin specific peptidase 25), also known as USP21, belongs to the peptidase C19 family and is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response (1). It contains one UBA-like domain and two UIM (ubiquitin-interacting motif) repeats. Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle-specific isoform (2). The long isoform of USP25 (USP25m) is upregulated in myogenesis and is implicated in regulation of muscular differentiation and function. USP25 is a deubiquitinating enzyme (DUB) that negatively regulates IL-17-triggered signaling (3,4).

    分子量

    Predicted: 116 kDa

    Observed: 105 kDa

    基因ID

    29761

    NCBI登录号

    NP_037528

    UniProt

    Q9UHP3
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