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alpha Tubulin 抗体 (N-Term)

This 兔 多克隆 antibody specifically detects alpha Tubulin in WB, ELISA, IF 和 IHC (p). It exhibits reactivity toward 人, 小鼠 和 大鼠.
产品编号 ABIN6991939
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for alpha Tubulin 抗体 (N-Term) (ABIN6991939)

抗原

See all alpha Tubulin (TUBA1) 抗体
alpha Tubulin (TUBA1)

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This alpha Tubulin antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

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    N-Term

    特异性

    Alpha-tubulin antibody is human, mouse and rat reactive.

    纯化方法

    Alpha-tubulin antibody is affinity chromatography purified via peptide column.

    免疫原

    Alpha-tubulin antibody was raised against an 18 amino acid peptide near the amino terminus of human alpha-tubulin. The immunogen is located within the first 50 amino acids of Alpha-tubulin.

    亚型

    IgG
  • 应用备注

    Alpha-tubulin antibody can be used for detection of alpha-tubulin by Western blot at 0.25 - 0.5 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

    Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Alpha-tubulin antibody is supplied in PBS containing 0.02 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C,4 °C

    储存方法

    Alpha-tubulin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
  • 抗原

    alpha Tubulin (TUBA1)

    别名

    Alpha-tubulin

    背景

    Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.

    分子量

    Predicted: 50 kDa

    Observed: 45 kDa

    基因ID

    7278

    NCBI登录号

    NP_005992

    UniProt

    Q13748

    途径

    Microtubule Dynamics
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