alpha Tubulin 抗体 (C-Term)
Quick Overview for alpha Tubulin 抗体 (C-Term) (ABIN6991938)
抗原
See all alpha Tubulin (TUBA1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- Alpha-Tubulin antibody is human, mouse and rat reactive.
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纯化方法
- alpha-Tubulin antibody is affinity chromatography purified via peptide column.
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免疫原
- alpha-Tubulin antibody was raised against an 18 amino acid peptide near the carboxy terminus of human alpha-Tubulin
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亚型
- IgG
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应用备注
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alpha-Tubulin antibody can be used for detection of alpha-Tubulin by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human, mouse, and rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- alpha-Tubulin antibody is supplied in PBS containing 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,4 °C
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储存方法
- alpha-Tubulin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
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- alpha Tubulin (TUBA1)
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别名
- alpha-Tubulin
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背景
- Alpha-Tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-Tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-Tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-Tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.
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分子量
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Predicted: 50 kDa
Observed: 54 kDa -
基因ID
- 7846
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途径
- Microtubule Dynamics
抗原
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