Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
特异性
IL-22 antibody is human, mouse, and rat reactive. Multiple isoforms of IL-22 are known to exist.
纯化方法
IL-22 antibody is affinity chromatography purified via peptide column.
免疫原
IL-22 antibody was raised against a 17 amino acid peptide near the center of human IL-22 . The immunogen is located within amino acids 90 - 140 of IL-22.
IL-22 antibody can be used for detection of IL-22 by Western blot at 0.5 μ,g/mL. Antibody can also be used for Immunocytochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
IL-22 antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
IL-22 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Interleukin-22 (IL-22) is a cytokine important for the modulation of tissue responses during inflammation (1). Unlike the distantly related IL-10, IL-22 does not inhibit the production of proinflammatory cytokines in monocytes in response to LPS, but it has some inhibitory effects on IL-4 production from Th2 T cells. IL-22 is expressed by both the adaptive arm of the immune system such as CD4 T cell subsets including Th17 cells, as well as by innate lymphocytes such as NK and LTi-like cells (2). IL-22 is highly expressed in several chronic inflammatory conditions, and studies suggest that IL-22 plays both inflammatory and protective roles (3).