DAP Antibody is affinity chromatography purified via peptide column.
免疫原
DAP antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DAP. The immunogen is located within the last 50 amino acids of DAP.
DAP
适用: 人, 大鼠
ELISA, IF (cc), IF (p), IHC (p), IHC (fro)
宿主: 兔
Polyclonal
unconjugated
应用备注
DAP antibody can be used for detection of DAP by Western blot at 1 - 2 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
DAP Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
DAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
抗原
DAP
(Death-Associated Protein (DAP))
别名
DAP
背景
DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15 kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51, under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2).