TRIM33 antibody is human, mouse and rat reactive. At least two isoforms of TRIM33 are known to exist.
纯化方法
TRIM33 Antibody is affinity chromatography purified via peptide column.
免疫原
TRIM33 antibody was raised against an 18 amino acid peptide near the center of human TRIM33. The immunogen is located within amino acids 580 - 630 of TRIM33.
TRIM33 antibody can be used for detection of TRIM33 by Western blot at 1 - 2 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
TRIM33 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
TRIM33 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
抗原
TRIM33
(Tripartite Motif Containing 33 (TRIM33))
别名
TRIM33
背景
TRIM33 Antibody: TRIM24 (TIF1alpha), TRIM28 (TIF1beta), and TRIM33 (TIF1gamma) are three related cofactors belonging to the tripartite motif superfamily that interact with distinct transcription factors (1-2). The structure of TRIM33 is similar to TRIM24 and TRIM28, exhibiting multiple domains (RING finger, coiled coil, B boxes, PHD/TTC, and bromodomain) (3). TRIM33 functions in cell differentiation and development, playing a role in differentiation of hematopoietic cells (3). It interacts with the Smad2/3 transcription factor in hematopoietic, mesenchymal, and epithelial cell types to mediate different transcriptional effects in response to TGF-beta (4). It acts as an E3 ubiquitin-protein ligase and promotes SMAD4 ubiquitination (5).