Western Blotting (WB), ELISA, Immunofluorescence (IF)
特异性
At least three isoforms of ACSL1 are known to exist, this antibody will detect all three isoforms.
纯化方法
ACSL1 Antibody is affinity chromatography purified via peptide column.
免疫原
Rabbit polyclonal ACSL1 antibody was raised against an 18 amino acid peptide near the center of human ACSL1. The immunogen is located within amino acids 240 - 290 of ACSL1.
ACSL1 antibody can be used for detection of ACSL1 by Western blot at 0.25 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
ACSL1 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
ACSL1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
抗原
ACSL1 (Acsl1)
(Acyl-CoA Synthetase Long-Chain Family Member 1 (Acsl1))
ACS1 antibody, FACL1 antibody, FACL2 antibody, LACS antibody, LACS1 antibody, LACS2 antibody, Acas antibody, Acas1 antibody, Acs antibody, FACS antibody, Facl2 antibody, ACS antibody, COAA antibody, zgc:110081 antibody, CER8 antibody, ECERIFERUM 8 antibody, LONG-CHAIN ACYL-COA SYNTHASE 1 antibody, T8I13.8 antibody, F13F21.14 antibody, F13F21_14 antibody, LATERAL ROOT DEVELOPMENT 2 antibody, LRD2 antibody, long-chain acyl-CoA synthetase 2 antibody, acyl-CoA synthetase long chain family member 1 antibody, acyl-CoA synthetase long-chain family member 1 antibody, acyl-CoA synthetase long chain family member 1a antibody, AMP-dependent synthetase and ligase family protein antibody, long-chain acyl-CoA synthetase 2 antibody, ACSL1 antibody, Acsl1 antibody, acsl1a antibody, LACS1 antibody, LACS2 antibody
背景
ACSL1 Antibody: Long-chain acyl coenzyme A synthetase 1 (ACSL1) catalyzes the synthesis of acyl-CoA from long-chain fatty acids in an ATP-dependent manner. ACSL1 is a member of a family of long-chain acyl-CoA synthetases which differ in substrate preference, tissue expression, and subcellular localization. In mouse, ACSL1 is the major acyl-CoA enzyme in the heart, providing 60-90 % of heart ATP. Loss of ACSL1 either globally or in heart ventricles resulted in impaired fatty acid oxidation, activation of the mammalian target of rapamycin (mTOR), and cardiac hypertrophy.