At least three isoforms of VAMP7 are known to exist, this antibody will detect the two longest isoforms. This antibody is predicted to not cross-react with other Ras-related proteins.
纯化方法
VAMP7 Antibody is affinity chromatography purified via peptide column.
免疫原
VAMP7 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human VAMP7. The immunogen is located within amino acids 20 - 70 of VAMP7.
VAMP7 antibody can be used for detection of VAMP7 by Western blot at 0.5 - 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
VAMP7 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
VAMP7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
抗原
VAMP7
(Vesicle-Associated Membrane Protein 7 (VAMP7))
别名
VAMP7
背景
VAMP7 Antibody: VAMP7 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family, localizing to late endosomes and lysosomes. VAMP7 is thought to mediate the fusion of endosomes to their target lysosomes as well as other exocytosis events during phagocytosis and neuritogenesis. VAMP7 interacts with the VPS9 ankyrin repeat protein VARP, a protein that localizes to early endosomes and thought to regulate endosome dynamics. Together with CD82, VAMP7 can modulate the signaling of EGFR by regulating its endocytosis from the plasma membrane.