Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
特异性
At least three isoforms of Thymopoietin are known to exist, this antibody will detect all isoforms. Thymopoietin antibody is predicted to not cross-react with other LEM domain-containing proteins.
纯化方法
Thymopoietin Antibody is affinity chromatography purified via peptide column.
免疫原
Thymopoietin antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human Thymopoietin. The immunogen is located within amino acids 80 - 130 of Thymopoietin.
Thymopoietin antibody can be used for detection of Thymopoietin by Western blot at 0.25 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 5 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
Thymopoietin Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
Thymopoietin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Thymopoietin Antibody: Thymopoietin (TMPO), also known as LAP2, is a widely expressed lem-domain-containing nuclear protein that is thought to play an important role in the regulation of nuclear architecture by binding lamin B1 and chromosomes in a manner regulated by phosphorylation during mitosis. Thymopoietin associates with nucleoplasmic lamins A/C, which are encoded by the dilated cardiomyopathy (DCM) gene LMNA, mutations in Thymopoietin disrupt this binding, suggesting that Thymopoietin may be a candidate disease gene for DCM.