ZNF219 抗体 (N-Term)
Our Local Distributor
北京 101111
Quick Overview for ZNF219 抗体 (N-Term) (ABIN6991505)
抗原
See all ZNF219 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 90-140, N-Term
-
特异性
- At least two isoforms of ZFP219 are known to exist, this antibody will recognize both.
-
纯化方法
- ZFP219 Antibody is affinity chromatography purified via peptide column.
-
免疫原
- ZFP219 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ZFP219. The immunogen is located within amino acids 90 - 140 of ZFP219.
-
亚型
- IgG
-
-
-
-
应用备注
-
ZFP219 antibody can be used for detection of ZFP219 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- ZFP219 Antibody is supplied in PBS containing 0.02 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C,4 °C
-
储存方法
- ZFP219 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
-
-
- ZNF219 (Zinc Finger Protein 219 (ZNF219))
-
别名
- ZFP219
-
背景
- ZFP219 Antibody: ZFP219 is a developmentally regulated member of the Kruppel-like zinc finger gene family that is thought to function as a transcriptional repressor. Yeast two-hybrid screening showed association with Sox9, a transcription factor that is essential for chondrogenesis. ZFP219 is specifically expressed in the developing limb buds and colocalizes with Sox9 in the nucleus. Knockdown of ZFP219 expression decreased Sox9-induced mRNA expression, and a dominant-negative mutant of ZFP219 inhibited Bmp2-induced chondrocyte differentiation, suggesting that ZFP219 plays an important role as a transcriptional partner of Sox9 in the regulation of chondrocyte differentiation.
-
分子量
-
Predicted: 80 kDa
Observed: 78 kDa -
基因ID
- 51222
-
NCBI登录号
- NP_001095142
-
UniProt
- Q9P2Y4
抗原
-