GLS2 抗体 (AA 300-350)
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- 抗原 See all GLS2 抗体
- GLS2 (Glutaminase 2 (Liver, Mitochondrial) (GLS2))
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抗原表位
- AA 300-350
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GLS2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
- 特异性
- Multiple isoforms of GLS2 are known to exist.
- 纯化方法
- GLS2 Antibody is affinity chromatography purified via peptide column.
- 免疫原
- GLS2 antibody was raised against an 18 amino acid synthetic peptide near the center terminus of human GLS2. The immunogen is located within amino acids 300 - 350 of GLS2.
- 亚型
- IgG
- Top Product
- Discover our top product GLS2 Primary Antibody
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anti-Glutaminase 2 (Liver, Mitochondrial) (GLS2) (AA 154-479) antibody
GLS2 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Glutaminase 2 (Liver, Mitochondrial) (GLS2) (Middle Region) antibodyGLS2 适用: 人, 小鼠, 大鼠, Pig, 犬, 斑马鱼, 马, 兔, Cow, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutaminase 2 (Liver, Mitochondrial) (GLS2) antibodyGLS2 适用: 人, 小鼠 WB, IHC (p) 宿主: 小鼠 Monoclonal 1758CT879-217-60 unconjugated
anti-Glutaminase 2 (Liver, Mitochondrial) (GLS2) (AA 241-290) antibodyGLS2 适用: 人, 小鼠, 大鼠, Pig, 犬, 斑马鱼, 马, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutaminase 2 (Liver, Mitochondrial) (GLS2) (AA 498-524) antibodyGLS2 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB20072 unconjugated
anti-Glutaminase 2 (Liver, Mitochondrial) (GLS2) (AA 41-140) antibodyGLS2 适用: 大鼠 ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Glutaminase 2 (Liver, Mitochondrial) (GLS2) (AA 15-602) antibodyGLS2 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutaminase 2 (Liver, Mitochondrial) (GLS2) (C-Term) antibodyGLS2 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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GLS2 antibody can be used for detection of GLS2 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in rat samples, Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- GLS2 Antibody is supplied in PBS containing 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C,4 °C
- 储存方法
- GLS2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- 抗原
- GLS2 (Glutaminase 2 (Liver, Mitochondrial) (GLS2))
- 别名
- GLS2 (GLS2 产品)
- 别名
- si:ch211-216k22.10 antibody, GA antibody, GLS antibody, LGA antibody, hLGA antibody, A330074B06Rik antibody, AI195532 antibody, Lga antibody, Ga antibody, glutaminase 2 antibody, glutaminase 2a (liver, mitochondrial) antibody, glutaminase 2 (liver, mitochondrial) antibody, glsA2 antibody, LOAG_03311 antibody, gls2a antibody, GLS2 antibody, Gls2 antibody
- 背景
- GLS2 Antibody: Phosphate-activated glutaminase, also known as Glutaminase 2 (GLS2), was initially isolated from rat liver, although it has been shown to be expressed in other tissues. Like the functionally similar, larger kidney glutaminase, GLS2 catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Expression of GLS2 is increased by p53 under both stressed and nonstressed conditions, resulting in increased levels of glutamate and alpha-ketoglutarate, which in turn results in enhanced mitochondrial respiration and ATP generation. GLS2 also regulates antioxidant defense function in cells by increasing reduced glutathione levels and decreasing ROS-levels, suggesting that GLS2 acts as a mediator of p53's role in antioxidant defense in addition to its role in energy metabolism.
- 分子量
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Predicted: 36, 55, 62, 66 kDa
Observed: 55, 62 kDa - 基因ID
- 27165
- NCBI登录号
- NP_037399
- UniProt
- Q9UI32
- 途径
- Dicarboxylic Acid Transport, Warburg Effect
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