Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
特异性
SPT2 antibody is predicted to not cross-react with SPT1.
纯化方法
SPT2 Antibody is affinity chromatography purified via peptide column.
免疫原
Anti-SPT2 antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human SPT2. The immunogen is located within the last 50 amino acids of SPT2.
Antibody validated: Western Blot in human and mouse samples, Immunocytochemistry in mouse samples, Immunofluorescence in mouse samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
SPT2 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
SPT2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
抗原
SPTLC2
(serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2))
SPT2 Antibody: Serine palmitoyltransferase (SPT), which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPT2 is the long chain base subunit 2 of mammalian serine palmitoyltransferase. SPT2 is catalytically active but needs its related protein SPT1 for its stabilization and anchoring the holoenzyme to the cytosolic face of the endoplasmic reticulum. As in the case with SPT1, mutations in the SPT2 gene can cause hereditary sensory and autonomic neuropathy type I (HSAN-I), resulting from a loss of SPT activity as well as the accumulation of the atypical and neurotoxic sphingoid metabolite 1-deoxy-sphinganine.