Western Blotting (WB), ELISA, Immunofluorescence (IF)
纯化方法
FEZ2 Antibody is affinity chromatography purified via peptide column.
免疫原
FEZ2 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human FEZ2. The immunogen is located within the last 50 amino acids of FEZ2.
FEZ2
适用: 大鼠, 小鼠
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
AbBy Fluor® 750
应用备注
FEZ2 antibody can be used for the detection of FEZ2 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunoflourescence starting at 5 μ,g/mL. For immunofluorescence start at 5 μ,g/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
FEZ2 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
FEZ2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
抗原
FEZ2
(Fasciculation and Elongation Protein zeta 2 (Zygin II) (FEZ2))
FEZ2 Antibody: Fasciculation and elongation protein zeta-2 (FEZ2) is a homolog to the mammalian FEZ1, itself an ortholog of the C. elegans UNC-76. In contrast to FEZ1, FEZ2 mRNA is widely expressed in mouse tissues. FEZ2 interacts with protein kinase C (PKC)-zeta substrate and induces neurite extension of PC12 cells when co-expressed with a constitutively active form of PKC-zeta, suggesting FEZ2 may play an important role in the morphological changes of various cells by associating with PKC-zeta in a tissue non-specific manner. FEZ2 can interact with FEZ1 through its c-terminal regions and especially its coiled-coil region. At least two isoforms of FEZ2 are known to exist.