At least two isoforms of STIM1 are known to exist, this antibody will detect only the larger form. This STIM1 antibody is predicted to have no cross-reactivity to STIM2.
纯化方法
STIM1 Antibody is affinity chromatography purified via peptide column.
免疫原
STIM1 antibody was raised against a 24 amino acid synthetic peptide from near the carboxy terminus of human STIM1. The immunogen is located within the last 50 amino acids of STIM1.
STIM1 antibody can be used for detection of STIM1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
STIM1 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
STIM1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
STIM1 Antibody: In T lymphocytes, the sole pathway for Ca2+ entry following antigen-receptor binding is through store-operated Ca2+-release-activated Ca2+ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca2+ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca2+ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca2+ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca2+ entry.