ERN1 抗体 (N-Term)
Quick Overview for ERN1 抗体 (N-Term) (ABIN6990592)
抗原
See all ERN1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 150-200, N-Term
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纯化方法
- IRE1p Antibody is affinity chromatography purified via peptide column.
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免疫原
- IRE1p antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human IRE1p. The immunogen is located within amino acids 150 - 200 of IRE1p.
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亚型
- IgG
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anti-Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (AA 401-500) antibody
ERN1 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 8D12 unconjugated
anti-Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (AA 401-500) antibodyERN1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3D1 unconjugated
anti-Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (AA 282-433) antibodyERN1 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 9F2 unconjugated
anti-Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (AA 252-260) antibodyERN1 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, FACS, IF (cc), IF (p), ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (pSer726) antibodyERN1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (pSer724) antibodyERN1 适用: 人 WB, ELISA, IF 宿主: 兔 Monoclonal 1F12 unconjugated Recombinant Antibody
anti-Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (Internal Region) antibodyVerified ERN1 适用: 人 WB, ELISA, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (C-Term) antibodyERN1 适用: 人, 小鼠, 大鼠 WB, IF, EIA 宿主: 兔 Polyclonal unconjugated
anti-Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (AA 493-561) antibodyERN1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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IRE1p antibody can be used for the detection of IRE1p by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 1 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- IRE1p Antibody is supplied in PBS containing 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,4 °C
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储存方法
- IRE1p antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))
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别名
- IRE1p
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背景
- IRE1p Antibody: Accumulation of malfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and the upregulation of the ER molecular chaperones GRP78 and GRP 94. These proteins are normally bound to ER transmembrane proteins such as IRE1p and ATF6 but ER stress causes their dissociation. This allows IRE1p, a serine-threonine protein kinase to transduce the unfolded protein signal from the ER to the nucleus. IRE1p also has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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基因ID
- 2081
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UniProt
- O75460
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途径
- ER-Nucleus Signaling, Unfolded Protein Response
抗原
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