PHAP III 抗体 (C-Term)
This anti-PHAP III antibody is a 兔 多克隆 antibody detecting PHAP III in WB, ELISA, IF 和 IHC (p). Suitable for 人, 小鼠 和 大鼠.
Quick Overview for PHAP III 抗体 (C-Term) (ABIN6990425)
抗原
PHAP III
适用
人, 小鼠, 大鼠
宿主
兔
克隆类型
多克隆
应用范围
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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抗原表位
- AA 200-250, C-Term
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特异性
- PHAP III has no cross-reaction to PHAP I and PHAP I2a.
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纯化方法
- PHAP III Antibody is affinity chromatography purified via peptide column.
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免疫原
- PHAP III antibody was raised with a synthetic peptide corresponding to amino acids close to carboxy terminus of human PHAP III. The immunogen is located within amino acids 200 - 250 of PHAP III.
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亚型
- IgG
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应用备注
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PHAP III antibody can be used for detection of PHAP III by Western blot at 1 μ,g/mL. A band at approximately 35 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.
Antibody validated: Western Blot in human and rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PHAP III Antibody is supplied in PBS containing 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,4 °C
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储存方法
- PHAP III antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- PHAP III
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背景
- PHAP III Antibody: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.
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分子量
- 35 kDa
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基因ID
- 81611
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NCBI登录号
- NP_112182
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UniProt
- Q9BTT0
抗原
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