IKAP/p150 抗体 (C-Term)
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北京 101111
Quick Overview for IKAP/p150 抗体 (C-Term) (ABIN6990353)
抗原
See all IKAP/p150 (ELP1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- At least two isoforms of IKAP are known two exist, this antibody will detect both isoforms.
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纯化方法
- IKAP Antibody is affinity chromatography purified via peptide column.
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免疫原
- IKAP antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human IKAP. The immunogen is located within the last 50 amino acids of IKAP.
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亚型
- IgG
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anti-Elongator Complex Protein 1 (ELP1) (AA 1107-1258) antibody
ELP1 适用: 人 WB, ELISA, IP, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Elongator Complex Protein 1 (ELP1) (AA 1242-1331) antibodyELP1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 6G9 unconjugated
anti-Elongator Complex Protein 1 (ELP1) (AA 1093-1332) antibodyELP1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Elongator Complex Protein 1 (ELP1) (C-Term) antibodyELP1 适用: 人, 小鼠 WB, IHC, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Elongator Complex Protein 1 (ELP1) (C-Term) antibodyELP1 适用: 人, 小鼠 WB, IF, EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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IKAP antibody can be used for detection of IKAP by Western blot at 0.5 to 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- IKAP Antibody is supplied in PBS containing 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,4 °C
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储存方法
- IKAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- IKAP/p150 (ELP1) (Elongator Complex Protein 1 (ELP1))
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别名
- IKAP
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背景
- IKAP Antibody: IKAP was initially identified as a scaffold protein of the Iκ,B kinase complex that could bind to IKKα, IKKβ, NF-κ,B, and the NF-κ,B-inducing kinase (NIK), although later evidence has cast doubt on this. More recent reports show that mutations in IKAP such as a frameshift leading to a truncated protein or a missense mutation that leads to defective phosphorylation are responsible for the autosomal recessive genetic disease familial dysautonomia (FD). Reports indicating that it forms part of the RNA polymerase II transcription elongation complex suggest that this disease may be due to compromised transcription elongation. More recently, it was shown that IKAP associates with c-Jun N-terminal kinase (JNK) and could specifically enhance JNK activation induced by the upstream JNK activators MEKK1 and ASK1, indicating another possible cause for FD.
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分子量
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Predicted: 134, 147 kDa
Observed: 105, 145 kDa -
基因ID
- 8518
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UniProt
- O95163
抗原
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