Ubiquitin is an important signaling protein that is catalytically added to proteins on lysine residues to modify their function. Addition of multiple ubiquitin proteins in a string (poly-ubiquitylation) to proteins signals their destruction by the proteasome. Mono-ubiquitylation of proteins has a much different effect, more in keeping with post-translational modification of proteins by phosphorylation or acetylation. Mono-ubiquitylation is part of a number of signal transduction pathways, including those involving histone proteins and transcriptional regulation. Ubiquitin antibody (mAb) (Clone P4D1) was raised in a Mouse host. It has been validated for use in Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
纯化方法
Protein A Chromatography
免疫原
This Ubiquitin antibody was raised against purified bovine ubiquitin.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
PBS, 30 % glycerol and 0.035 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.