COVID-19, which is short for coronavirus disease 2019, is the official name of the respiratory disease caused by infection with the novel coronavirus SARS-CoV-2. The virus that causes COVID-19 was named SARS-CoV-2 because it is a coronavirus genetically similar to, yet distinct from, the virus that caused the severe acute respiratory syndrome (SARS) outbreak in 2003. Studying the details of how this virus replicates and causes the disease will allow scientists and physicians to more rapidly develop fast and accurate methods of detection as well as to deploy therapeutic and vaccine strategies. This antibody was derived from COVID-19 patients who have cleared the virus. Patient serum IgG was sequenced and expressed as a chimeric full-length human immunoglobulin in mammalian 293 cells. This chimeric antibody contains a heavy chain CH1 that is IgG1 isotype, whereas its CH2/CH3/CH4 regions are IgM isotype. The antibody contains a Flag Tag and an 8X His Tag at the C-terminus. SARS-CoV-2 Spike IgG/IgM Antibody (AM5) (Clone AM5 (105-43)) was raised in a Human host. It has been validated for use in ELISA, it has been shown to react with Virus samples.
纯化方法
Ni-NTA
免疫原
Derived from COVID-19 patients who have cleared the virus. Their antibodies were screened for reactivity to SARS-CoV-2 and then sequenced and expressed as recombinant antibodies.
克隆位点
AM043105 (105-43)
亚型
IgG, IgM
应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
PBS, 30 % glycerol, 0.035 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.