POLR2F 抗体
Quick Overview for POLR2F 抗体 (ABIN6972612)
抗原
See all POLR2F 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. RNA pol II antibody (mAb) (Clone 1F4B6) was raised in a Rat host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples.
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纯化方法
- Protein G Chromatography
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免疫原
- This antibody was raised against a synthetic peptide containing the CTD of human RNA Polymerase II.
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亚型
- IgG2a
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anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 1-127) antibody
POLR2F 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 2G2 unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (N-Term) antibodyPOLR2F 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) antibodyPOLR2F 适用: 人, 大鼠, 小鼠, C. elegans, Saccharomyces cerevisiae WB, IF, ChIP, ChIP-seq, ICC 宿主: 小鼠 Monoclonal 4H8 unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 27-61) antibodyPOLR2F 适用: 小鼠 WB 宿主: 兔 Polyclonal RB56843 unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) antibodyPOLR2F 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 1-127) antibodyPOLR2F 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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- POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))
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别名
- RNA pol II
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分子量
- 240 kDa
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NCBI登录号
- NP_000928
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途径
- Regulatory RNA Pathways
抗原
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