Histone H4 抗体 (acLys12)
Quick Overview for Histone H4 抗体 (acLys12) (ABIN6972105)
抗原
See all Histone H4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- acLys12
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产品特性
- Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. The acetylation of histone H4 Lys12 is carried out by several histone acetyltransferases (HATs). Histone H4 Lys12 acetylation by TIP60 occurs in the nucleus, whereas Hat1-mediated acetylation of histone H4 Lys12 occurs in the cytoplasm, indicating the spatial regulation of histone acetylation. The acetylation of histone H4 Lys12 residue is commonly associated with transcriptional activation. Histone H4K12ac antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, Dot blot, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Budding Yeast, Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.
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纯化方法
- Protein A Chromatography
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免疫原
- This Histone H4 acetyl Lys12 antibody was raised against a peptide including acetyl-lysine 12 of histone H4.
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亚型
- IgG
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anti-Histone H4 (acLys12) antibody
适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (3meLys20) antibody适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (acLys12) antibody适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (acLys5) antibody适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Histone H4 (meLys20) antibody适用: 人 WB, IHC, IF, DB 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (2meLys20) antibody适用: 人 WB, IHC, IF, IP, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (3meLys20) antibody适用: 人 WB, IHC, IF, DB 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (acLys8) antibody适用: 人 WB, IHC, IF, IP, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (acLys16) antibody适用: 人, Saccharomyces cerevisiae, Drosophila melanogaster WB, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (acLys16) antibody适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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缓冲液
- Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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- Histone H4
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分子量
- 8 kDa
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NCBI登录号
- NP_778224
抗原
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