Histone H3.1 抗体 (AA 21-39)
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北京 101111
Quick Overview for Histone H3.1 抗体 (AA 21-39) (ABIN6971835)
抗原
See all Histone H3.1 (HIST1H3B) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 21-39
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产品特性
- Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. There are three protein variants of Histone H3, Histone H3.1, 3.2 and 3.3. The incorporation of Histone H3.1 and H3.2 into nucleosomes is replication dependent, in contrast to Histone H3.3, which is independent of DNA synthesis and occurs throughout the cell cycle. Human Histone H3.1 and H3.2 are identical in amino acid sequences except at position 110 where H3.1 has a cysteine and H3.2 has a serine. Histone H3.1 / 3.2 antibody (mAb) (Clone 1D4F2) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
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纯化方法
- Protein A Chromatography
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免疫原
- This antibody was raised against a peptide comprising amino acids 21-39 of human Histone H3.1. This region is 100% identical in human Histone H3.2.
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亚型
- IgG2b
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anti-Histone H3.1 (HIST1H3B) antibody
HIST1H3B 适用: 人 WB, IF, ELISA, IHC, FACS 宿主: 兔 Monoclonal 27F2 unconjugated Recombinant Antibody
anti-Histone H3.1 (HIST1H3B) (pSer10) antibodyHIST1H3B 适用: 人, 小鼠, 大鼠 WB, IF, ELISA, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Histone H3.1 (HIST1H3B) (H3K4me2) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, ICC, FACS 宿主: 兔 Monoclonal 6F6 unconjugated Recombinant Antibody
anti-Histone H3.1 (HIST1H3B) (H3S10ph) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Histone H3.1 (HIST1H3B) (meArg17) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, ICC 宿主: 兔 Monoclonal 3E10 unconjugated Recombinant Antibody
anti-Histone H3.1 (HIST1H3B) (H3S10ph) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, ICC 宿主: 兔 Monoclonal 2A5 unconjugated Recombinant Antibody
anti-Histone H3.1 (HIST1H3B) (H3R2me1) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, ICC 宿主: 兔 Monoclonal 4G5 unconjugated Recombinant Antibody
anti-Histone H3.1 (HIST1H3B) (meLys36) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, ICC 宿主: 兔 Monoclonal 1F4 unconjugated Recombinant Antibody
anti-Histone H3.1 (HIST1H3B) (H3K14ac) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, ICC 宿主: 兔 Monoclonal 3H3 unconjugated Recombinant Antibody
anti-Histone H3.1 (HIST1H3B) (H3K18me1) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, ICC 宿主: 兔 Monoclonal 2B5 unconjugated Recombinant Antibody
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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- Histone H3.1 (HIST1H3B)
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别名
- Histone H3.1 / 3.2
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分子量
- 17 kDa
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NCBI登录号
- NP_003522
抗原
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