Bub1 is a serine-threonine protein kinase and an important regulator of cell division. It is essential for the G2/M mitotic spindle assembly/integrity checkpoint as well as critical for chromosome alignment. Bub1 is required for the assembly of a large number of proteins at the kinetochore, including CENP-E, CENP-F, BUB1B, MAD2L1 and PLK1. It plays a role in sister chromatid cohesion and it serves as a substrate for the anaphase-promoting complex. Bub1 mediates cell death in response to chromosome mis-segregation and acts to suppress spontaneous tumorigenesis. Bub1 antibody (mAb) (Clone 14H5) was raised in a Mouse host. It has been validated for use in Immunocytochemistry and Immunofluorescence, it has been shown to react with Human and Mouse samples.
纯化方法
Protein G Chromatography
免疫原
This Bub1 antibody was raised against a recombinant protein corresponding to amino acids 281-419 of human Bub1.
BUB1
适用: 小鼠, 大鼠
IHC, IF
宿主: 兔
Polyclonal
unconjugated
应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
抗原
BUB1
(Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))