Argonaute 3 (Ago3, aka EIF2C3) is a member of the Argonaute family of proteins involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Ago3, however, lacks RNase activity and does not appear to cleave target mRNA molecules. Ago3 has both a PAZ and a PIWI domain, indicative of the Argonaute family of proteins. Ago3 antibody (mAb) (Clone 4B1-F6) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, Immunocytochemistry, Immunofluorescence and Immunoprecipitation, it has been shown to react with Human samples.
纯化方法
Protein G Chromatography
免疫原
This Ago3 (Argonaute 3) antibody was raised against a recombinant protein corresponding to amino acids 1-175 of human Ago3.
EIF2C3
适用: Cow, 斑马鱼
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
Cy7
应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.