IL-31 抗体
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北京 101111
Quick Overview for IL-31 抗体 (ABIN6964030)
抗原
See all IL-31 (IL31) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Native and recombinant human IL-31
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纯化方法
- Purified from in vitro cultures by protein G affinity chromatography.
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免疫原
- Recombinant human IL-31
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亚型
- IgG1
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应用备注
- Neutralization of human IL-31 bioactivity. It is recommended to establish the optimal antibody concentration for the assay system used. Neutralization Neutralization assay: Neutralization of human IL-31 induced IL-6 production (see graph). To determine the neutralizing capacity and ND50, mixtures of human recombinant IL-31 (50 ng/ ml) and various concentrations of mAb MT313 were added to human aortic endothelial cells that had been pre-stimulated with recombinant human IFN-γ (10 ng/mL). Following overnight incubation, cell supernatants were quantified in IL-6 ELISA. Neutralization dose 50 (ND50): MT313 neutralized the effect of human IL-31 at a ND50value of 0.1-0.6 μg/mL when 50 ng/mL of human IL-31 was present. ND50 is defined as the concentration of antibody that results in 50 % inhibition of the biological response under the experimental conditions specified.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- supplied at 1 mg/mL in PBS free of preservatives
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
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有效期
- 18 months
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- IL-31 (IL31) (Interleukin 31 (IL31))
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别名
- IL31
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基因ID
- 386653
抗原
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