(1 reference)-
- 抗原 See all IL-12/IL-23 p40 products
- IL-12/IL-23 p40
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This IL-12/IL-23 p40 antibody is un-conjugated
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应用范围
- Neutralization (Neut)
- 特异性
- Native and recombinant p40 subunit of human IL-12 and IL-23
- 纯化方法
- Purified from in vitro cultures by protein G affinity chromatography.
- 免疫原
- Recombinant human IL-23
- 克隆位点
- MT3279H
- 亚型
- IgG2b
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anti-IL-12/IL-23 p40 antibody (APC)
适用: 小鼠 FACS 宿主: 大鼠 Monoclonal C17-8 APC
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- 应用备注
- Neutralization of human IL-12 and IL-23 bioactivity. It is recommended to establish the optimal antibody concentration for the assay system used. Neutralization Neutralization assay: Neutralization of human IL-12 and human IL-23 induced IFN-γ production (see graphs). To determine the neutralizing capacity and ND50 on IL-12 induced IFN-γ production, mixtures of various concentrations of mAb MT3279H, human recombinant IL-12 (10 ng/mL) and recombinant IL-1β (50 ng/mL) were added to human PBMC. For determination of the neutralizing capacity and ND50 on IL-23 induced IFN-γ production, mixtures of various concentrations of mAb MT3279H, human recombinant IL-23 (10 ng/ ml) and recombinant IL-18 (50 ng/mL) were added to human PBMC. Following overnight incubation, cell supernatants were quantified in IFN-γ ELISA. Neutralization dose 50 (ND50): MT3279H neutralized the effect of IL-12 and IL-23 at a ND50value of 0.2-0.5 μg/mL when 10 ng/mL of recombinant human IL-12 or IL-23 was present. ND50 is defined as the concentration of antibody that results in 50 % inhibition of the biological response under the experimental conditions specified.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- supplied at 1 mg/mL in PBS free of preservatives
- 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
- 有效期
- 18 months
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: "Epstein-Barr virus coinfection in children boosts cytomegalovirus-induced differentiation of natural killer cells." in: Journal of virology, Vol. 87, Issue 24, pp. 13446-55, (2014) (PubMed).
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: "Epstein-Barr virus coinfection in children boosts cytomegalovirus-induced differentiation of natural killer cells." in: Journal of virology, Vol. 87, Issue 24, pp. 13446-55, (2014) (PubMed).
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- 抗原
- IL-12/IL-23 p40
- 别名
- IL12/23 (p40) (IL-12/IL-23 p40 产品)
- 基因ID
- 3592, 3593
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