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APOE 抗体 (Biotin)

This anti-APOE antibody is a 小鼠 单克隆 antibody detecting APOE in WB, ELISA 和 ELISpot. Suitable for 人 和 Non-Human Primate. This Primary Antibody has been cited in 5+ publications.
产品编号 ABIN6963938
发货至: 中国

Quick Overview for APOE 抗体 (Biotin) (ABIN6963938)

抗原

See all APOE 抗体
APOE (Apolipoprotein E (APOE))

适用

  • 110
  • 86
  • 74
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
人, Non-Human Primate

宿主

  • 124
  • 45
  • 9
  • 2
  • 1
小鼠

克隆类型

  • 122
  • 58
单克隆

标记

  • 98
  • 17
  • 11
  • 6
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
This APOE antibody is conjugated to Biotin

应用范围

  • 145
  • 69
  • 60
  • 47
  • 44
  • 39
  • 39
  • 22
  • 13
  • 12
  • 9
  • 6
  • 5
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, ELISpot

克隆位点

E887
  • 特异性

    Native and recombinant apolipoprotein E (isoforms apoE2, apoE3 and apoE4).

    交叉反应 (详细)

    The monoclonal antibody cross-reacts with apoE from non-human primates.

    纯化方法

    Purified from in vitro cultures by protein G affinity chromatography.

    免疫原

    Purified human apolipoprotein E

    亚型

    IgG1
  • 应用备注

    For quantification of apoE in serum/plasma samples and cell culture supernatants using ELISA and for enumeration of human apoE producing cells using ELISpot. E887 is recommended as detection mAb in combination with coating mAb E276 . The antibody is also suitable for immunoprecipitation and Western blot.

    说明

    Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

    有效期

    18 months
  • Bissig-Choisat, Wang, Legras, Saha, Chen, Bell, Pankowicz, Hill, Barzi, Leyton, Leung, Kruse, Himes, Goss, Wilson, Chan, Lagor, Bissig: "Development and rescue of human familial hypercholesterolaemia in a xenograft mouse model." in: Nature communications, Vol. 6, pp. 7339, (2015) (PubMed).

    Bankwitz, Vieyres, Hueging, Bitzegeio, Doepke, Chhatwal, Haid, Catanese, Zeisel, Nicosia, Baumert, Kaderali, Pietschmann: "Role of hypervariable region 1 for the interplay of hepatitis C virus with entry factors and lipoproteins." in: Journal of virology, Vol. 88, Issue 21, pp. 12644-55, (2014) (PubMed).

    Braesch-Andersen, Paulie, Smedman, Mia, Kumagai-Braesch: "ApoE production in human monocytes and its regulation by inflammatory cytokines." in: PLoS ONE, Vol. 8, Issue 11, pp. e79908, (2014) (PubMed).

    Handattu, Nayyar, Garber, Palgunachari, Monroe, Keenum, Mishra, Datta, Anantharamaiah: "Two apolipoprotein E mimetic peptides with similar cholesterol reducing properties exhibit differential atheroprotective effects in LDL-R null mice." in: Atherosclerosis, Vol. 227, Issue 1, pp. 58-64, (2013) (PubMed).

    Liu, Kuhel, Shen, Hui, Woods: "Apolipoprotein E does not cross the blood-cerebrospinal fluid barrier, as revealed by an improved technique for sampling CSF from mice." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 303, Issue 9, pp. R903-8, (2013) (PubMed).

  • 抗原

    APOE (Apolipoprotein E (APOE))

    别名

    apoE

    基因ID

    348

    途径

    Regulation of Cell Size, Lipid Metabolism
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