IL-4 抗体 (PromoFluor-488 Premium)
Quick Overview for IL-4 抗体 (PromoFluor-488 Premium) (ABIN6963827)
抗原
See all IL-4 (IL4) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Native IL-4
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交叉反应 （详细）
- The monoclonal antibody cross-reacts with IL-4 from non-human primates.
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纯化方法
- Purified from in vitro cultures by protein G affinity chromatography.
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免疫原
- Recombinant human IL-4
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亚型
- IgG1
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anti-Interleukin 4 (IL4) (AA 25-153) antibody
IL4 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Interleukin 4 (IL4) (AA 25-153) antibodyIL4 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (AA 122-150) antibodyIL4 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (AA 61-111) antibodyIL4 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Interleukin 4 (IL4) (AA 25-153) antibodyIL4 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (Internal Region) antibodyIL4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (AA 122-150), (AA 122-151), (C-Term) antibodyIL4 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB22885 unconjugated
anti-Interleukin 4 (IL4) antibodyIL4 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (AA 26-136) antibodyIL4 适用: 小鸡 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) antibody (PE)IL4 适用: 人, 猕猴 FACS 宿主: 大鼠 Monoclonal MP4-25D2 PE
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应用备注
- Detection of IL-4 producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IL-4 by flow cytometry in viable human PBMC. Cells were stimulated for 16 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were stained with anti-CD3-BV421, fixed and permeabilized using 4 % paraformaldehyde and saponin, and further stained with PF488P-conjugated mAb IL4-3 (right panel). Non-stimulated cells shown as reference (left panel). The dot plot derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- in PBS with 0.2 % BSA and 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
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有效期
- 18 months
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- IL-4 (IL4) (Interleukin 4 (IL4))
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别名
- IL4
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基因ID
- 3565
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
抗原
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