IL-2 抗体 (PromoFluor 647 Premium)
Quick Overview for IL-2 抗体 (PromoFluor 647 Premium) (ABIN6963756)
抗原
See all IL-2 (IL2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Native and recombinant IL-2
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交叉反应 （详细）
- The monoclonal antibody cross-reacts with IL-2 from sheep. For dection of IL-2 in sheep, unconjugated MT3B3 with second step detection is recommended.
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纯化方法
- Purified from in vitro cultures by protein G affinity chromatography.
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免疫原
- Recombinant bovine IL-2
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亚型
- IgG1
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anti-Interleukin 2 (IL2) (AA 21-153) antibody
IL2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 (IL2) (AA 21-153) antibodyIL2 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Interleukin 2 (IL2) (AA 10-169) antibodyIL2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 (IL2) (AA 21-153) antibodyIL2 适用: 人 WB 宿主: 小鼠 Monoclonal C5 unconjugated
anti-Interleukin 2 (IL2) antibodyIL2 适用: 人 WB, ELISA, IP, ICC, FACS 宿主: 小鼠 Monoclonal 35C3 unconjugated
anti-Interleukin 2 (IL2) antibody (APC)IL2 适用: 人 ICFC 宿主: 小鼠 Monoclonal 35C3 APC
anti-Interleukin 2 (IL2) antibody (PE)IL2 适用: 人 FACS 宿主: 小鼠 Monoclonal 35C3 PE
anti-Interleukin 2 (IL2) antibodyIL2 适用: 人 WB, ELISA, FACS 宿主: 兔 Monoclonal unconjugated
anti-Interleukin 2 (IL2) (AA 21-155) antibodyIL2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 (IL2) (AA 25-176) antibodyIL2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Detection of IL-2 producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IL-2 by flow cytometry in viable bovine PBMC. Cells were stimulated for 5 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with anti- bovine IL-2 mAb MT3B3-PF647P (solid line). Matched isotype control antibody MT128. The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- in PBS with 0.2 % BSA and 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
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有效期
- 18 months
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- IL-2 (IL2) (Interleukin 2 (IL2))
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别名
- IL2
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基因ID
- 280822
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
抗原
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