CD8 alpha 抗体 (PE-Cy7)
Quick Overview for CD8 alpha 抗体 (PE-Cy7) (ABIN7278393)
抗原
See all CD8 alpha (CD8A) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.
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亚型
- IgG2a
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anti-CD8a Molecule (CD8A) (AA 22-235) antibody
CD8A 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 8H8A8 unconjugated
anti-CD8a Molecule (CD8A) (AA 51-150) antibodyCD8A 适用: 人, 大鼠, 小鸡 FACS, WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 人 IHC, WB, IP, ELISA 宿主: 兔 Monoclonal 2F2 unconjugated Recombinant Antibody
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 小鼠 FACS, IP, IHC (fro), IHC (p), ICC, Func 宿主: 大鼠 Monoclonal 53-6-7 unconjugated
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 人 IHC, StM, ISt 宿主: 小鼠 Monoclonal C8-468 unconjugated
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 小鼠 FACS, IP, IHC (fro), IHC (p), ICC 宿主: 大鼠 Monoclonal 53-6-7 unconjugated
anti-CD8a Molecule (CD8A) (AA 22-182) antibodyCD8A 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 6A9A5 unconjugated
anti-CD8a Molecule (CD8A) (AA 28-196) antibodyCD8A 适用: 小鼠, 大鼠 FACS, IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD8a Molecule (CD8A) (AA 150-180) antibodyCD8A 适用: 人 FACS, IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD8a Molecule (CD8A) (AA 59-88) antibodyCD8A 适用: 人 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indi- cated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system.
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说明
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5 uL (0.25 ug)/test Human peripheral blood lymphocytes were stained with 5 uL (0.25 ug)
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限制
- 仅限研究用
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缓冲液
- 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- 2-8°C protected from light
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有效期
- 12 months
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- CD8 alpha (CD8A) (CD8a Molecule (CD8A))
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别名
- CD8a
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背景
- The OKT8 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodi- mer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitat- ing intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites. The OKT8 an- tibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. If used together with alternative antibodies Anti-Human CD8a clone RPA-T8 or Anti-Human CD8a clone Hit8a, the OKT8 antibody will not block binding of RPA-T8 or Hit8a.
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基因ID
- 925
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UniProt
- P01732
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途径
- TCR Signaling
抗原
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