Recombinant mRuby 抗体
Quick Overview for Recombinant mRuby 抗体 (ABIN6953292)
抗原
抗体类型
片段
适用
宿主
克隆类型
标记
应用范围
克隆位点
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Expression System
- E.coli
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原理
- Unlabelled anti-mRuby sdAb with ectopic Cysteine on N- and C-Term for maleimide conjugation.
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特异性
- MRUBY3 and mRUBY derivatives
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产品特性
- The anti-mRUBY single-domain antibody has the same specificity as the respective labeled variant, but can be purchased here as unconjugated version.
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纯化方法
- Produced in: E.coli
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Affinity
- Extinction coefficient (1/M*cm): 20190
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anti-mRuby antibody (Atto 488)
适用: Entacmaea quadricolor IF, ICC, FACS 宿主: Alpaca Monoclonal 1F8 Atto 488 single-domain Antibody (sdAb) Recombinant Antibody
anti-mRuby antibody适用: Discosoma IF, IEM, IHC (fro), IHC (p), WB 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Uncoupled sdAb for doing maleimide conjugations. You can chose the position of the ectopic cysteine (N-terminal, C-terminal or both ends).
Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only. -
实验流程
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Maleimide Labelling Protocol
- Remove TCEP and exchange buffer to 10 mM KPi, 300 mM NaCl, 2 mM EDTA, pH 6.0 by size exclusion chromatography
- Before labelling, add 1/10 vol 1M Tris/HCl pH 8.0
- On ice, add 1.5- to 5- fold molar excess of fluorophore as maleimide
- Quickly vortex
- Overlay with Argon
- Place on ice for 90 min
- Separate excess of dye from the labelled sdAb e.g. by size exclusion chromatography
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Unconjugated sdAbs are lyophilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA. Reconstitute with 250 µL pure water. Use unconjugated sdAbs immediately after reconstitution.
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缓冲液
- lyophilized from 10 mM KPi, 300 mM NaCl, 2 mM EDTA, pH 6.0
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储存条件
- -20 °C,-80 °C
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储存方法
- Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below
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有效期
- 12 months
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- mRuby
抗原
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