Recombinant mScarlet 抗体 (Alexa Fluor 647)
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- 抗原
- mScarlet
- 抗体类型
- Recombinant Antibody
- 片段
- single-domain Antibody (sdAb)
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适用
- Dictyosoma
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宿主
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Alpaca
- Expression System
- E.coli
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克隆类型
- 单克隆
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标记
- Alexa Fluor 647
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应用范围
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
- 原理
- Camelid sdAb anti-mScarlet-i conjugated with Alexa647, Clone 2B12
- 特异性
- Recognizes mScarlet-I and most fluorescent proteins from Dictiosoma sp. and most common RFP derivatives
- 交叉反应 （详细）
- Does not cross-react with GFP, CFP, YFP and mTagBFP or their most common derivatives.
- 产品特性
- A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
- 纯化方法
- Produced in: E.coli
- 克隆位点
- 2B12
- Labeling Ratio
- Two site-specifically conjugated fluorophores per sdAb.
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anti-mScarlet antibody
适用: Discosoma IF, IEM, IHC (fro), IHC (p), WB 宿主: 山羊 Polyclonal unconjugated
anti-mScarlet antibody (Atto 488)适用: Dictyosoma IF, FACS, ICC 宿主: Alpaca Monoclonal 2B12 Atto 488 single-domain Antibody (sdAb) Recombinant Antibody
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- 应用备注
- Recommended dilution 1:500
- 说明
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Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- 5 μM
- 缓冲液
- 2.5 μM fluorescently labeled sdAb in buffered saline, 50 % glycerol, 0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from light!
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below
- 有效期
- 12 months
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- 抗原
- mScarlet
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