Cullin 4A 抗体
Quick Overview for Cullin 4A 抗体 (ABIN6942738)
抗原
See all Cullin 4A (CUL4A) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
- 
    - 
                                            交叉反应
- 人
- 
                                            纯化方法
- Purified by Protein A.
- 
                                            免疫原
- Recombinant protein within human Cullin 4a aa 600-759
- 
                                            亚型
- IgG
 
- 
                                            
- 
    
- 
    - 
                                            应用备注
- 
                        WB 1:300-5000
 FCM 1:20-100
 IHC-P 1:200-400
 IF(IHC-P) 1:50-200
 IF(ICC) 1:50-200
- 
                                            限制
- 仅限研究用
 
- 
                                            
- 
    - 
                                            状态
- Liquid
- 
                                            浓度
- 1 μg/μL
- 
                                            缓冲液
- Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
- 
                                            储存液
- ProClin
- 
                                            注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 
                                            储存条件
- 4 °C,-20 °C
- 
                                            储存方法
- Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
- 
                                            有效期
- 12 months
 
- 
                                            
- 
    - Cullin 4A (CUL4A)
- 
                                            别名
- CUL4A
- 
                                            背景
- 
                        Synonyms: Cullin-4A, CUL-4A, CUL4A, Background: CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins. CUL4A is a core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. DCX(DTL) directs autoubiquitination of DTL. 
- 
                                            基因ID
- 8451
- 
                                            UniProt
- Q13619
 抗原
- 
                    
 
                                     
                                     
                                    