CRISPR-Cas9 (AA 1-200) 抗体
-
- 抗原
- CRISPR-Cas9
-
抗原表位
- AA 1-200
-
适用
- 其他
-
宿主
- 兔
-
克隆类型
- 单克隆
-
标记
- 非结合性
-
应用范围
- Western Blotting (WB)
- 交叉反应
- 人
- 交叉反应 （详细）
- Streptococcus pyogenes
- 纯化方法
- Purified by Protein A.
- 免疫原
- CRISPR-Cas9 between 1-200 amino acids
- 克隆位点
- 6C1
- 亚型
- IgG
-
anti-CRISPR-Cas9 (AA 1-462) antibody
适用: Staphylococcus aureus WB 宿主: 小鼠 Monoclonal unconjugated Recombinant Antibody
anti-CRISPR-Cas9 (C-Term) antibody适用: Streptococcus pyogenes WB, ELISA, FM 宿主: 兔 Polyclonal unconjugated
anti-CRISPR-Cas9 antibody适用: Staphylococcus aureus WB, IF 宿主: 兔 Polyclonal unconjugated
anti-CRISPR-Cas9 (C-Term) antibody (HRP)适用: Streptococcus pyogenes WB, ELISA, FM 宿主: 兔 Polyclonal HRP
anti-CRISPR-Cas9 (C-Term) antibody (Biotin)适用: Streptococcus pyogenes WB, ELISA, FM 宿主: 兔 Polyclonal Biotin
anti-CRISPR-Cas9 antibody适用: Streptococcus pyogenes WB, IF, IP, ChIP 宿主: 小鼠 Monoclonal 8C1-F10 unconjugated
anti-CRISPR-Cas9 antibody适用: 人 WB 宿主: 小鼠 Monoclonal 1E8 unconjugated
anti-CRISPR-Cas9 (C-Term) antibody (DyLight 800)适用: Streptococcus pyogenes WB, ELISA, FM 宿主: 兔 Polyclonal DyLight 800
anti-CRISPR-Cas9 (C-Term) antibody (DyLight 549)适用: Streptococcus pyogenes WB, ELISA, FM 宿主: 兔 Polyclonal DyLight 549
-
- 应用备注
- WB(1:300-1000)
- 限制
- 仅限研究用
-
- 浓度
- 1 μg/μL
- 缓冲液
- Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C for 12 months.
- 有效期
- 12 months
-
- 抗原
- CRISPR-Cas9
- 别名
- CRISPR-Cas9 SP
- 背景
-
Synonyms: CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9, cas9
Background: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids) (PubMed:21455174). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA, Cas9 only stabilizes the pre-crRNA:tracrRNA interaction and has no catalytic function in RNA processing (PubMed:24270795). Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer, Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed 3'-5' exonucleolytically. DNA-binding requires protein and both gRNAs, as does nuclease activity. Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. DNA strand separation and heteroduplex formation starts at PAM sites, PAM recognition is required for catalytic activity (PubMed:24476820). Confers immunity against a plasmid with homology to the appropriate CRISPR spacer sequences (CRISPR interference) (PubMed:21455174).
- 基因ID
- 901176
- UniProt
- Q99ZW2
-
