Recombinant Cytokeratin, Multi (Epithelial Marker) 抗体
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- 抗原
- Cytokeratin, Multi (Epithelial Marker)
- 抗体类型
- Recombinant Antibody
- 适用
- Cow, Frog, 山羊, 豚鼠, 人, 猴, 小鼠, Pig, 大鼠
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宿主
- 兔
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克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- Immunohistochemistry (IHC), Staining Methods (StM), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
- 特异性
- Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13) and 45 kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Keratin-enriched preparation from cultured human epithelial cells
- 克隆位点
- KRT-1877R
- 亚型
- IgG
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anti-Cytokeratin, Multi (Epithelial Marker) antibody
适用: Cow, Frog, 山羊, 豚鼠, 人, 猴, 小鼠, Pig, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal rKRT-457 unconjugated Recombinant Antibody
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- 应用备注
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Positive Control: A431 cells, Skin, Colon carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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