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Keratin 77 抗体

This anti-Keratin 77 antibody is a 小鼠 单克隆 antibody detecting Keratin 77 in IHC 和 StM. Suitable for 人 和 大鼠.
产品编号 ABIN6941447
发货至: 中国

Quick Overview for Keratin 77 抗体 (ABIN6941447)

抗原

See all Keratin 77 (KRT77) 抗体
Keratin 77 (KRT77)

适用

  • 19
  • 6
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  • 2
  • 1
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人, 大鼠

宿主

  • 12
  • 7
小鼠

克隆类型

  • 10
  • 9
单克隆

标记

  • 15
  • 2
  • 1
  • 1
This Keratin 77 antibody is un-conjugated

应用范围

  • 11
  • 9
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Immunohistochemistry (IHC), Staining Methods (StM)

克隆位点

KRTL-1077
  • 特异性

    This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

    交叉反应 (详细)

    Shows broad species reactivity.

    纯化方法

    Purified by Protein A/G

    免疫原

    Recombinant human KRT77 protein

    亚型

    IgG1 kappa
  • 应用备注

    Positive Control: Skin, Squamous cell carcinoma (SCC).

    Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    Keratin 77 (KRT77)

    别名

    KRT77

    分子量

    56.5kDa (CK10), 50kDa (CK14), 50kDa (CK15), 48kDa (CK16), 40kDa (CK19)

    基因ID

    374454

    UniProt

    Q7Z794
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